Group training on the production of sterilized substrates and lignicolous mushrooms

Next edition: 20th - 24th November 2017. Location: at Mycelia’s premises, Veldeken 38A 9850 Nevele, Belgium

Considering the succes of the previous editions, we are now proposing the 12th group training at our premises, which will cover all aspects of lignicolous mushroom cultivation. 

Over the years, Mycelia has acquired an excellent reputation as a training centre for mycelium and lignicolous substrate producers. Since November 2011, we have developed our group trainings on the production of sterilized substrates and lignicolous mushrooms, which cover the whole production cycle from mother culture to specialty mushroom.

The program has been developed in close collaboration with dipl. Ing. Jürgen Kynast, who has a broad-spectrum scientific background in combination with an extensive experience as a grower of specialty mushrooms. We welcome existing and would-be growers to this in-depth course. 


Sunday 19/11/2017: 

Arrival at the B&B, or the hotel of your choice. 

Collection at the train station will be organized.

  • In the morning, Magda will be at Mycelia for the last preparations, while driving to the train station to collect the participants. Other pickup arrangements can be made, but should be communicated clearly.
  • In the afternoon she will be at Mycelia or in De Steenhove to prepare didactic materials and meet the participants.


Monday 20/11/2017: 

8h15 - 8h30

walk to Mycelia

8h30 - 8h40

welcome and practical briefing

8h40 - 9h30

parallel session - introduction 

     - 25 min

group 1: quiz slides

     - 25 min

group 2: Mycelia floor plan and visit to the premises

9h30 - 10h

coffee break

10h - 11h30

first introduction to substrate production: components, mixing, humidification, filling, bag closing, sterilization, cooling, spawning, incubation, growing systems (Jürgen)

11h30 - 12h30

parallel session – practical tests

      - 30 min

group 1: agar (Pascale + Hélène)

     - test with petridishes: testing hands and air

     - making agar

      - 30 min

group 2: make your own substrate (Kasper)

12h30 - 13h30

lunch break

13h30 - 14h30

biology part I: (Magda)

     - general biology

     - role of enzymes

14h30 - 15h

coffee break

15h00 - 16h

parallel session - production lab research

     - 20 min

group 1: applied microscopy (Kasper)

     - 20 min

group 2: visit to technical floor with overpressure units (Magda)

     - 20 min

group 3: pouring agar under LAF (Hélène)

16h - 17h

biology part II: bacteria and fungi (Magda)

     - bacteria: cell structure and multiplication

     - perfect and imperfect Fungi: classification and life cycle


Tuesday 21/11/2017: 

8h15 - 8h30

walk to Mycelia

8h30 - 9h00

quiz (Magda)

9h - 12h30

parallel session - ingredients and consumables

     - 60 min

group 1: practical substrate preparation in Mycelia’s workshop (Jean & Pascale)

     - selection of ingredients
     - filling of mixer
     - addition of water
     - examples of filling and mixing machines
     - pH and dry matter measurement

10h - 10h30

coffee break

     - 60 min

group 2: improving the structure of substrates (Jürgen)

     - determination of water- and air-filled pores
     - characteristics and role of different additives: wood chips, wood shavings etc
     - examples of substrate formulations on paper

     - 60 min

group 3: demonstration of incubating substrates (Kasper)

     - good and bad examples
     - discussion

12h30 - 13h30

lunch break

13h30 - 15h00

working in cleanrooms (Magda):

     - presence of propagules in the air
     - cleanroom classes, air quality control
     - use of LAF, use of overpressure
     - rules of conduct

15h - 15h30

coffee break

15h30 - 16h30

biological approach of different production stages (Jürgen): incubation, ripening, fructification

16h30 - 17h

incubation and ripening phases: climate conditions, temperature vs. time (Jürgen)


Wednesday 22/11/2017: 

8h15 - 8h30

walk to Mycelia

8h30 - 10h

pests and diseases: prevention and solutions (Jürgen)

10h - 10h40

spawn (Magda):  

     - spawn production and spawn quality
     - spawn testing
     - transport and conservation

10h40 - 11h

coffee break

11h - 12h30

autoclaving process (Jürgen)

     - theory on steam production and heat transfer
     - correct autoclaving procedure
     - F- value
     - what happens in the substrate bags?

12h30 - 13h30

lunch break

13h30 - 15h30

parallel session - practical inoculation, machinery

     - 40 min

group 1: practical substrate inoculation in Mycelia’s technical lab (Magda & Jürgen)

     - essentials of small-scale production inoculation
     - particle counting and air speed measurement

     - 40 min

group 2: practical substrate inoculation in Mycelia’s cleanroom (Hélène & Kasper)

essentials of large-scale production inoculation

     - 40 min

group 3: autoclaves and steam generator (Pascale)

     - practical use of steam (Pascale)
     - valve and sterilisation cycle schematics

15h30 - 16h

coffee break

16h15 - 17h

theory of pasteurization process (Jürgen)


Thursday 23/11/2017: 

8h15 - 8h30

walk to Mycelia

8h30 - 9h20

production technics (Jürgen):

     - cooling and heating (Jürgen)
     - racks and shelves (Jürgen)

9h30 - 10h30:

breathing bags: techniques and gas exchange (Jan)

10h30 - 10h50

coffee break

10h50 - 11h50

parallel session

     - 30 min   

group 1: construction and maintenance of laminar air flows (Kasper)

     - filter types, practicalities, dangers
     - practical review of LAF’s
     - build and efficiency

     - 30 min   

group 2: cleanroom consumables and dressing procedure (Magda)

11h50 - 13h00

fructification techniques (Jürgen):

     - aeration systems
     - humidification systems
     - hygiene in fructification room
     - harvesting, mushroom quality
     - post-harvest care
     - spent substrate

13h00 - 14h30

lunch break

14h30 - 15h15

fructification phase: determination of best climate conditions (Jürgen)

15h15 - 15h45

coffee break

15h45 - 16h

group activity - 3 groups (Jürgen):

     - make the layout of a substrate annex mushroom production farm, taking into account all information which you have received

     - group discussion

16h - 16h45

building technics (Jürgen):

     - construction materials

     - water supply, waste water

     - electricity, energy efficiency

16h45 - 17h

evaluation of petridishes made on Monday


Friday 24/11/2017: 


meeting outside the B&B De Steenhove (Veldeken 45, 9850 Nevele)

6h45 - 9h

drive to Martijn Van Herwaarden (Luttel Inghweg 12, Kerkdriel 5331 PN)

9h - 12h30

visit to Martijn van Herwaarden  (Luttel Inghweg 12, Kerkdriel 5331 PN): Pleurotus substrate producer and grower of Shiitake and Pleurotus

12h30 - 13h

drive to Paddestoelenrijk (Middelweg 3, Rossum 5328 GL): grower of various types of lignicolousmushrooms

13h - 14h

lunch break – lignicolous mushroom tasting, delivery of USB stick and certificate and closing of the course.

14h - 15h30

visit to the Paddestoelenrijk (Middelweg 3, Rossum 5328 GL)

15h30 - 18h

drive back to Belgium

If you wish to participate, please take contact with us via email:


  • 1180 EUR/ participant, ex VAT, board and lodging not included.
  • our students’ rate, 20% reduction to the full tariff, is 944 EUR. Please add a copy of your students’ card to the registration form.

Included are:

  • bread lunch Monday until Thursday, at “De Steenhove”. The last lunch, on Friday in the restaurant in Germany, is not included.
  • welcome evening and dinner on Monday night, with visit to the beautiful city of Gent.
  • transport to and back from the dutch growers.

Payment: a pro forma invoice will be made to the company and address data on your registration form. We kindly ask prepayment not later than the 1st November.


  • Cancellation before 13/11/2017: 100% refunding, minus bank costs.
  • Cancellation after 13/11/2017: 50% refunding, minus bank costs.